Synthesis, Characterization and DNA Cleavage of Copper(II) Complex with D,L-Dithiothreitol
نویسندگان
چکیده
Purpose: To study deoxyribonucleic acid (DNA) shearing capability of copper(II) complex of dithiothreitol (DTT) and to fevaluate its potential application in cancer therapy. Methods: A parrot green complex was synthesized by grinding copper acetate monohydrate and DTT in 1:2 molar ratio in a mortar until no fumes of acetic acid were observed. The complex was characterized using attenuated total reflectance-Fourier transform infra-red (ATR-FTIR), and x-ray diffraction (XRD) techniques. Further information was also collected through Karl Fischer titration, thermogravimetric analysis (TGA) and (magnetic moment. Cleavage of DNA was determined by agarose gel electrophoresis. The gel was then stained, analyzed and photographed under ultraviolet (UV) light. Results: ATR-FTIR confirmed the formation of copper(II) complex with DTT by binding through thiol group based on the disappearance of the thiol (-SH) stretching peak at 2545 cm. The crystalline structure was elucidated by a sharp intense peak at 38.520 in XRD spectrum while the octahedral geometry of complex was inferred from a magnetic moment of 1.72 B.M. The results for water content obtained by Karl Fischer titration and TGA revealed that water molecules are not part of the coordination sphere of the complex. Cleavage study of DNA showed that the complex completely sheared the circular DNA compared to pure DTT. Conclusion: Solvent free synthesis of Copper(II)-DTT complex has been successfully achieved, and an anhydrous complex with octahedral geometry obtained. The complex has a greater potential to shear DNA molecule than pure DTT.
منابع مشابه
Biologically vital metal-based antimicrobial active mixed ligand complexes: synthesis, characterization, DNA binding and cleavage studies
Few novel cobalt(II) and copper(II) complexes [M(fmp)3]Cl2, [M(fmp)(bpy)2]Cl2,[M(fmp)(phen)2]Cl2 and [M(fmp)(phen)(bpy)]Cl2 (fmp = 3-furan-2-ylmethylene-pentane-2,4-dione, phen = 1,10-phenanthroline, bpy = 2,2'-bipyridine) have been synthesized andcharacterized by elemental analyses, molar conductance, magnetic susceptibility measurements,IR, electronic, EPR, mass spectra and cyclic voltammetri...
متن کاملDNA interaction and antimicrobial studies of novel copper (II) complex having ternary Schiff base
A novel ternary Schiff base ligand (HL) of ONO type and its copper (II) complex weresynthesised using 2-aminophenol and o-acetoacetotoluidide. They have been characterised by theusual analytical and spectral methods. The interaction of the complex with calf-thymus (CT)DNA has been investigated by UV-Vis, viscosity measurement, cyclic voltammetry anddifferential pulse voltammetry studies. The re...
متن کاملSynthesis, characterization and selective oxidation using a new copper (II) Schiff base complex derived from Alanine and 4-chloro3- formyl coumarin
A novel Schiff-base ligand (L: 2-[(4-chloro-2-oxo-2H-chromen-3-ylmethylene)-amino] propionic acid) was prepared from the reaction of 4-chloro3-formylcoumarin and alanine amino acid. Copper (II) complex was synthesized from the reaction of the ligand with Cu (OAc)2. H2O in ethanol. The ligand and its metal complex were characterized by elemental analysis (CHN), ICP, thermal analysis (TGA), Fouri...
متن کاملSynthesis, Characterization and Crystal Structure Determination of Copper (II) Complexes with 2,2′-Dimethyl-4,4′-bithiazole
Copper(II) complex [Cu(dmbt)2(H2O)](ClO4)2 (1) was prepared from the reaction of copper(II) perchlorate hexahydrate with 2,2'-dimethyl-4,4'-bithiazole (dmbt) ligand in methanol at ambient temperature. The complex was quantitatively and qualitatively characterized by elemental analysis, absorption and infrared spectrometries. Complex [Cu(DMSO)5](ClO4)2 (2) was also synt...
متن کاملSynthesis, Spectroscopy and Magnetic Characterization of Five Dinuclear Copper(II) Complexes with 2, 3 or 4-Pyridinemethanol as the Ligand
The synthesis, spectroscopy and magnetic characterization of five new dinuclear copper(II) complexes are described. All five compounds have the general formula [Cu2(L)4(O-R)2](Cl)2 or [Cu2(L)2(Cl)2](Cl)2, in which R = CH3 or H, L=2, 3 or 4-pyridinemethanol as L2, L3 or L4, respectively. The title compounds consist of dinuclear units with bridging methoxo groups in [Cu2(L4)4(O-CH3)2](Cl)2, hydro...
متن کامل